2024年2月25日发(作者：horizon)

人参研究GINSENGRESEARCH2023年第4期DOI院10.19403/.1671-1521.2023.园4.011

35HPLC摘赵宁宁1袁魏文增2渊1.福建医科大学实验动物中心袁福州350122曰2.福建省中医药科学院袁福州350003冤ODS2min袁袁关键词院要院HPLC遥Hypersil(4.6mm伊250mm袁5滋m)袁-渊35颐65袁V颐V冤袁1.0mL/306nm袁20滋L遥300~12.5滋mol/L0.9991袁98.78%袁(RSD)9.13%遥尧袁遥曰曰曰尧尧尧[6-10]遥渊resveratrol袁Res冤袁袁C14H12O3渊228.25袁[1]袁[2-4]袁[5]遥Res尧尧尧尧3袁4'袁5-1冤袁袁尧尧尧尧尧尧袁尧作者简介院图1Res的化学结构图Res袁Res[11,12]袁遥Res袁袁袁[13,14]遥袁袁[15]遥袁[16,17]遥HPLC袁Res遥1仪器与材料1.1Waters渊Waters冤曰MQD-S3渊冤曰FA124C袁袁袁袁渊袁d=0.0001g冤曰BK-180J渊渊冤冤曰XH-C渊冤曰DW-86L626渊冤曰DW-25L262渊冤曰HAC-I渊冤遥1.2Res渊M17HB178543冤袁曰NADPH渊411U022冤袁曰袁20mg/mL渊M10011冤袁曰渊207899冤渊204127冤袁Fisher遥2方法与结果2.1袁HypersilODS2渊4.6mm伊250mm袁5滋m冤曰-渊35颐65袁V颐V冤曰306nm曰1.0mL/min曰20滋L遥2.22.2.1Res袁袁袁25滋mol/L袁遥2.2.2NADPHNADPH袁PBS袁袁10mmol/L袁遥2.3500滋L渊3冤袁10滋L尧NADPH20滋L尧PBS500滋L袁Res37益5min袁5滋LRes渊1%冤袁37益袁遥2.4遥

36赵宁宁等院HPLC法测定白藜芦醇在大鼠肝微粒体体外代谢含量人参研究GINSENGRESEARCH2023年第4期

袁500滋L袁3min15min渊15000r/min冤袁袁袁袁袁遥2.52.5.1袁2遥图2Res及其大鼠肝微粒体代谢的HPLC色谱图渊袁A.空白组渊无Res冤袁标准品溶液袁孵育代谢组冤2.5.2渊100益10min冤Res袁300尧200尧100尧50尧2512.5滋mol/L袁野2.32.4冶袁野2.1冶袁袁渊Y冤袁Res渊X冤袁遥Y=46660X-228300渊r=0.9991冤袁Res300耀12.5滋mol/L遥2.5.3尧尧Res渊25尧100300滋mol/L冤袁野2.32.4冶袁野2.1冶袁3d袁遥ResRSD1.65%尧0.85%尧0.82%袁3dRSD10.94%袁遥2.5.4野2.5.3冶袁0尧2尧4尧6尧8h袁遥ResRSD10.54%袁8h遥2.5.5野2.5.3冶袁3袁遥ResRSD10.33%袁遥2.5.6野2.5.3冶袁遥Res尧尧102.52%尧99.34%尧94.49%袁RSD9.13%尧3.17%尧3.79%袁遥2.6野2.32.4冶袁6h袁野2.1冶袁袁遥Res6h袁1遥

人参研究GINSENGRESEARCH2023年第4期表1Res

反应6h后的代谢含量结果渊n=3冤渊滋mol/L冤38.2943.2298.579210.6539.078.89232.9245.848.10211.2944.768.723结论与讨论3.1HPLCRes袁HPLC院-渊35颐65袁V颐V冤曰306nm曰1.0mL/min曰20滋L遥HPLC尧尧尧尧袁Res遥3.2HPLC遥袁袁Res306nm遥306nm遥3.3HPLC遥-袁-袁Res遥-袁Res袁遥-遥3.4袁尧袁[18]遥ResHPLC袁袁Res袁Res袁袁Res袁Res袁Res遥参考文献[1]张兰胜袁刘光明.白藜芦醇的研究概述[J].大理学院学报袁2007袁6渊4冤:72-74.[2]LIUWei,DINGXiaoxia,LIPeiwu,za鄄tionofpretreat-mentmethodsfordeterminationofresver鄄atrolinpeanut[J].JournalofShanxiAgriculturalSciences,2017,45(10):1695-1697.[3]XIAOLinxia,LUQineng,chprogressofresveratrol[J].ModernAgriculturalScienceandTechnology,2015(24):264-265.[4]RENPing袁licKineticsofResveratrolinLiverMicrosomalEnzymeofRats[J].JournalofHubeiUniversityofScienceandTechnology(MedicalSciences),2013,27(1):5-8.[5]HANJingjing,LIUWei,esinresveratrolstudies[J].ChineseJournalofBiotechnol鄄ogy,2008,24(11):1851-1859.[6]JanaJaklov佗Dytrtov佗,MichalStraka,KaterinaB侑lonon侏kov佗,sveratrolretainitsantiox鄄idativepropertiesinwineRedoxbehaviourofresveratrolinthepresenceofCu(II)andtebuconazole[J].FoodChemistry袁2018袁262(1):221-225援[7]JiaRui,LiYao,CaoLiping,idative,an鄄ti-inflammatoryandhepatoprotectiveeffectsofresvera鄄trolonoxidativestress-inducedliverdamageintilapia(Oreochromisniloticus)[J].ComparativeBiochemistryandPhysiology,PartC,2019,215(1):56-66援[8]贾文斌袁钱跹袁吕磊.薄层色谱法和高效液相色谱法在白藜芦醇胶囊定性定量研究中的应用[J].海军医学杂志袁2017袁38(6):506-508援[9]LukaszHuminiecki袁JaroslawHorba俳ctionalgenomicstudiesofresveratrolinrespecttoitsanti-cancereffects[J].BiotechnologyAdvances袁2018袁36(1):1699-1708援[10]黎永胜袁文军.白藜芦醇的药理作用研究进展[J].医学综述袁2008袁14(3):469.[11]周丽袁董永海袁胡传来袁等.HPLC法测定葡萄汁(酒)中的白藜芦醇和白藜芦醇苷[J].营养学报袁2010袁32渊1冤:86-87(+90).[12]张瑞袁汪电雷袁张弦袁等.高效液相色谱法测定大鼠血浆中白藜芦醇含量及其药物代谢动力学[J].安徽中医学院学报袁2010袁29渊5冤:56-58.[13]WALLET袁HSIEHF袁DELEGGEMH袁sorptionbutverylowbioavailabilityoforalresveratrolinhumans[J].DrugMetabDispos袁2004袁32(12):1377-1382.[14]GESCHERAJ,onshipbe鄄tweenmechanisms,bioavailibility,andpreclinicalchemo鄄preventiveefficacyofresveratrol:Aconundrum[J].CancerEpidemiolBiomarkersPrev,2003,12(10):953-957.[15]谈恒山袁张海霞袁方芸袁葛卫红.近10年国内药物体外代谢研究进展[J].江苏药学与临床研究袁2006袁14渊5冤院297-298.[16]RawdenHC,CarlileDJ,TindallA,o鄄malPerdietionofinvivoClearanceandassociatedin鄄terindividualvabilityofsixbenzodiazepinesinhumans[J].Xenobiotiea,2005,35:603-625.[17]YangZ,ZadjuraL,D'ArienzoC,nicalpharmacokineticsofanovelHIV-1attachmentinhibitorBMS-378806andperdictionofitshumanphmara鄄cokinet-es[J].BiopharmDrugDispos,2005,26:387-402.[18]WeiWenzeng,HuangHuiling,YeiLixiang,liskineticsandsubtypeanalysisofCYP450ofkoumineinlivermicrosomesofhuman,minipig,rat,mon鄄keyanddog[J].JournalofFujianMedicalUniversity,2017,51(2):82-87.37