2024年4月11日发(作者：)

e3泛素连接酶组成

英文回答：

E3 ubiquitin ligase complex:

E3 ubiquitin ligases are responsible for the final step

of ubiquitination, the covalent attachment of ubiquitin to

a target protein. They are composed of multiple subunits,

including:

RING finger domain-containing proteins: These proteins

contain a RING finger domain, which binds to the catalytic

domain of the E2 enzyme and facilitates ubiquitin transfer

to the target protein.

HECT domain-containing proteins: These proteins contain

a HECT (homologous to E6AP C-terminus) domain, which first

binds to ubiquitin and then transfers it to the target

protein.

Cullin-based E3 ligases: These ligases are composed of

a cullin protein, which forms the core of the complex, and

a variety of other subunits, including a RING finger

protein and a substrate-binding protein.

E3 ubiquitin ligases can be classified into two types:

Monomeric E3 ligases: These ligases consist of a single

polypeptide chain. They typically contain both a RING

finger domain and a substrate-binding domain.

Multimeric E3 ligases: These ligases are composed of

multiple subunits. They typically contain a RING finger

protein, a HECT domain protein, or a cullin protein, as

well as one or more substrate-binding subunits.

中文回答：

E3泛素连接酶组成：

E3泛素连接酶负责泛素化的最后一步，将泛素共价连接到靶蛋

白上。它们由多个亚基组成，包括：

RING指结构域含有的蛋白质，这些蛋白质包含RING指结构域，

它结合到E2酶的催化结构域，促进泛素转移到靶蛋白。

HECT结构域含有的蛋白质，这些蛋白中包含HECT（与E6AP C

末端同源）结构域，该结构域先结合泛素，然后将其转移到靶蛋白。

基于cullin的E3连接酶，这些连接酶由cullin蛋白组成，它

们构成了该复合物的核心，以及多种其他亚基，包括RING指蛋白和

底物结合蛋白。

E3泛素连接酶可分为两类：

单体型E3连接酶，这些连接酶由单个多肽链组成。它们通常同

时包含RING指结构域和底物结合结构域。

多聚体E3连接酶，这些连接酶由多个亚基组成。它们通常包含

RING指蛋白、HECT结构域蛋白或cullin蛋白，以及一个或多个底

物结合亚基。