2024年4月11日发(作者：)

黑曲霉发酵生产糖化酶的工艺流程

English Answer:

Black Aspergillus Fermentation for the Production of

Cellulase.

Process Flow Diagram:

1. Strain Selection and Preparation: Select a suitable

strain of Black Aspergillus for cellulase production.

Prepare the inoculum by culturing the strain in a suitable

growth medium.

2. Substrate Preparation: Prepare a substrate

containing cellulosic materials, such as agricultural

residues (e.g., corn stover, wheat straw), or industrial

byproducts (e.g., paper pulp, wood chips).

3. Fermentation: Inoculate the prepared substrate with

the Black Aspergillus inoculum. Conduct fermentation under

optimal conditions for cellulase production, including

temperature, pH, aeration, and agitation.

4. Enzymatic Extraction: After fermentation, extract

the cellulase enzymes from the fermented substrate using

suitable extraction techniques, such as mechanical

disruption, solvent extraction, or enzymatic hydrolysis.

5. Enzyme Purification: Optionally, purify the

extracted cellulases to improve their activity and

specificity. This can be achieved through techniques such

as chromatography, precipitation, or membrane filtration.

6. Formulation and Application: Formulate the cellulase

enzymes into a suitable product form for specific

applications, such as in the paper and pulp industry,

biofuel production, or textile manufacturing.

Factors Influencing Cellulase Production:

Substrate Composition: The composition and structure

of the cellulosic substrate significantly impact cellulase