2024年4月13日发(作者：)

4%琼脂糖凝胶电泳

英文回答：

4% Agarose Gel Electrophoresis.

4% agarose gel electrophoresis is a widely used

technique in molecular biology to separate and analyze DNA

fragments based on their size. It involves running an

electric current through a gel made of agarose, a

polysaccharide derived from seaweed. The negatively charged

DNA fragments migrate towards the positive electrode, with

smaller fragments moving faster than larger fragments. By

comparing the migration distance of the DNA fragments to a

DNA ladder of known sizes, the size of the DNA fragments

can be estimated.

Materials.

4% agarose gel.

DNA samples.

DNA ladder.

Electrophoresis buffer (e.g., TBE or TAE buffer)。

Electrophoresis chamber.

Power supply.

Gel documentation system.

Procedure.

1. Prepare a 4% agarose gel by dissolving 4 grams of

agarose powder in 100 mL of electrophoresis buffer.

2. Heat the agarose solution in a microwave or on a hot

plate until it boils and becomes clear.

3. Pour the molten agarose solution into a gel casting

tray and insert a comb to create wells for the DNA samples.