2023年7月25日发(作者：)

限制性内切酶 兼容粘性末端Compatible Cohesive Ends matinlda

Enzyme

Acc65I

(G/GTACC)

Ligated To

BanI (G/GTACC)

BsiWI, BsrGI

Recleaved By

Acc65I, BanI, KpnI, NlaIV, RsaI

RsaI

--

TaqαI

--

AciI

HpaII

--

AccI

AciI, AclI, BsaHI (GR/CGYC), HinP1I, HpaII, NarI

ClaI, BstBI, TaqαI

AccI (GT/CGAC), AclI, ClaI, BstBI, TaqαI

BsaHI (GR/CGCC), HinP1I, NarI

HpaII

(GT/CGAC)

(GT/CGAC)

AciI

(C/CGC)

AclI

(AA/CGTT)

AgeI

(A/CCGGT)

AccI (GT/CGAC), AciI, ClaI, BstBI, HinP1I, HpaII, NarI, TaqαI

AvaI (C/CCGGG), XmaI

BsaWI, BspEI

BsrFI (A/CCGGT), SgrAI (CA/CCGGTG)

NgoMIV

HpaII, NciI, ScrFI

BsaWI, HpaII

AgeI, BsaWI, BsrFI, HpaII

BsrFI, HpaII

ApaI, BanII, Bsp120I, Bsp1286I, HaeIII, NlaIV,

Sau96I

ApaI

(GGGCC/C)

ApaLI

(G/TGCAC)

ApoIBanII (GGGCC/C), Bsp1286I (GGGCC/C)

SfcI (C/TGCAG) BsgI

EcoRI

EcoRI

MfeI, Tsp509I

AflIII (A/CGCGT), MluI

BssHII

ApoI, Tsp509I

ApoI, EcoRI, Tsp509I

Tsp509I

BstUI, HhaI

BssHII, BstUI, Cac8I, HhaI

--

MseI

(A/AATTY)

(G/AATTY)

(R/AATTY)

AscI

(GG/CGCGCC)

AseI

(AT/TAAT)

BfaI, Csp6I, NdeI

MseI Enzyme

AsiSI

(GCGAT/CGC)

Ligated To

BsiEI (CGAT/CG)

PacI

PvuI

Recleaved By

DpnII, PvuI

MseI

DpnII, PvuI

HpaII, NciI, ScrFI

AvaI

AgeI, BsaWI, BspEI, BsrFI (R/CCGGY), NgoMIV, SgrAI

(CR/CCGGYG)

(C/CCGGG)

(C/TCGAG)

(C/TCGAG)

(C/CCGGG)

AvaII

(G/GWCC)

XhoI

SalI

XmaI

PpuMI (RG/GACCY)

RsrII

PpuMI (RG/GTCCY)

AvaI, TaqαI, XhoI

TaqαI

AvaI, BsaJI, HpaII, NciI, ScrFI, SmaI

AvaII, NlaIV, Sau96I

AvaII, Sau96I

AvaII, BsmFI, NlaIV, Sau96I

BfaI

AvrII, BfaI, BsaJI, StyI

AlwI, DpnII

AlwI, BstYI, DpnII

AlwI, BamHI, BstYI, DpnII, NlaIV

Acc65I, BanI, KpnI, NlaIV, RsaI

BanI, BsaHI, HaeII, HhaI, KasI, NarI, NlaIV

RsaI

ApaI, BanII, Bsp1286I, HaeIII, NlaIV, Sau96I

AluI, BanII, BsiHKAI, Bsp1286I, SacI

AlwI, DpnII

DpnII

AvrII

(C/CTAGG)

NheI, SpeI, XbaI

StyI (C/CTAGG)

BamHI

(G/GATCC)

BclI, DpnII

BglII, BstYI (R/GATCY)

BstYI (G/GATCC)

BanI

Acc65I

KasI

BsiWI, BsrGI

ApaI, Bsp1286I (GGGCC/C)

Bsp1286I (GAGCT/C), SacI

BamHI, BstYI (R/GATCY)

BglII, MboII

(G/GTACC)

(G/GCGCC)

(G/GTACC)

BanII(GGGCC/C)

(GAGCT/C)

BclI

(T/GATCA) Enzyme

BfaI

(C/TAG)

BglII

(A/GATCT)

Ligated To

AseI, Csp6I, MseI, NdeI

Recleaved By

--

BamHI, BstYI (R/GATCY)

BclI, DpnII

AlwI, BstYI, DpnII

DpnII

--

HgaI

HhaI

AciI

BsaHI, HgaI

BanI, BsaHI, HaeII, HhaI, NarI, NlaIV

AgeI, BsaWI, BsrFI, HpaII

HpaII, NciI, ScrFI

BsaWI, BspEI, HpaII

BsrFI, HpaII

HpaII

MseI

BsiEI, DpnII, PvuI

AciI

BsiHKAI, Bsp1286I

BsiHKAI, Bsp1286I

AluI, BanII, BsiHKAI, Bsp1286I, SacI

--

BsgI

RsaI

BsaHI

AccI (GT/CGAC), ClaI, BstBI, TaqαI

AciI, HinP1I

AciI, HinP1I

HpaII

NarI

NarI

AgeI, BsrFI (R/CCGGY), SgrAI (CR/CCGGYG)

AvaI (C/CCGGG), XmaI

BspEI

BsrFI (R/CCGGY), NgoMIV

NgoMIV

(GR/CGYC)

(GA/CGYC)

(GG/CGYC)

(GG/CGYC)

(GA/CGYC)

(GG/CGYC)

BsaWI

(W/CCGGW)

BsiEI

PacI

PvuI

SacII

Bsp1286I (GTGCA/C)

Bsp1286I (GAGCA/C)

Bsp1286I (GAGCT/C), SacI

NsiI

PstI, SbfI

(CGAT/CG)

(CGAT/CG)

(CGGC/CG)

BsiHKAI

(GTGCA/C)

BsiWI

(C/GTACG)

Acc65I, BanI (G/GTACC), BsrGI Enzyme

Bsp1286ILigated To

ApaI, BanII (GGGCC/C)

BsiHKAI

BanII (GGGCC/C)

BanII (GAGCT/C), BsiHKAI, SacI

BsiHKAI

NsiI

PstI, SbfI

AgeI, BsaWI, BsrFI (R/CCGGY), SgrAI (CR/CCGGYG)

AvaI (C/CCGGG), XmaI

BsaWI

BsrFI (R/CCGGY), NgoMIV

Recleaved By

ApaI, BanII, Bsp1286I, HaeIII, NlaIV, Sau96I

ApaLI, BsiHKAI, Bsp1286I

BanII, Bsp1286I

AluI, BanII, BsiHKAI, Bsp1286I, SacI

BsiHKAI, Bsp1286I

--

BsgI

BsaWI, HpaII

HpaII, NciI, ScrFI

BsaWI, BspEI, HpaII

HpaII

FatI, NlaIII

(GGGCC/C)

(GTGCA/C)

(GGGCC/C)

(GAGCT/C)

(GWGCW/C)

(GTGCA/C)

(GTGCA/C)

BspEI

(T/CCGGA)

BspHI

(T/CATGA)

BsrFIFatI, NcoI, PciI

AgeI, BsaWI

AgeI, BsaWI, NgoMIV

AvaI (C/CCGGG), XmaI

BsaWI, BspEI

BsaWI, BspEI

NgoMIV

SgrAI

Acc65I, BanI (G/GTACC), BsiWI

AgeI, BsaWI, BsrFI, HpaII

BsrFI, HpaII

HpaII, NciI, ScrFI

BsaWI, HpaII

HpaII

BsrFI, Cac8I, HpaII, NaeI

BsrFI, HpaII

RsaI

(A/CCGGY)

(G/CCGGY)

(R/CCGGY)

(A/CCGGY)

(R/CCGGY)

(G/CCGGY)

(CR/CCGGYG)

BsrGI

(T/GTACA)

BssHII

(G/CGCGC)

MluI

AscI

BstUI, HhaI

BssHII, BstUI, Cac8I, HhaI

TaqI BstBI AccI (GT/CGAC), ClaI, TaqI Enzyme

(TT/CGAA)

BstYILigated To

AciI, AclI, BsaHI (GR/CGYC), HinP1I, HpaII, NarI

BamHI, BglII

BamHI

BclI, DpnII

BclI, DpnII

BglII

AccI (GT/CGAC), BstBI, TaqI

AciI, AclI, BsaHI (GR/CGYC), HinP1I, HpaII, NarI

Recleaved By

--

AlwI, BstYI, DpnII

AlwI, BamHI, BstYI, DpnII, NlaIV

DpnII

AlwI, DpnII

BglII, BstYI, DpnII

TaqI

--

AlwI, DpnII

DpnII

HaeIII, Sau96I

BsiEI, EaeI, EagI, HaeIII

EaeI, HaeIII

AciI, BsiEI, EaeI, EagI, Fnu4HI, HaeIII

AciI, EaeI, Fnu4HI, HaeIII

HaeIII, Sau96I

EaeI, HaeIII

BsiEI, EaeI, EagI, HaeIII

AciI, BsiEI, EaeI, EagI, Fnu4HI, HaeIII

ApoI, EcoRI, Tsp509I

ApoI, Tsp509I

Tsp509I

FatI, NlaIII

(A/GATCY)

(G/GATCY)

(R/GATCY)

(G/GATCY)

(A/GATCY)

ClaI

(AT/CGAT)

DpnII/MboI/

Sau3AI (/GATC)

BamHI, BstYI (R/GATCC)

BclI, BglII, BstYI (R/GATCY)

EaeI

PspOMI

EagI

EagI

NotI

NotI

PspOMI

EaeI (Y/GGCCR)

EaeI (C/GGCCG)

NotI

(Y/GGCCR)

(C/GGCCR)

(T/GGCCR)

(C/GGCCR)

(T/GGCCR)

EagI

(C/GGCCG)

EcoRI

(G/AATTC)

ApoI (G/AATTC)

ApoI (R/AATTY)

MfeI, Tsp509I

FatI

(/CATG)

BspHI, NcoI, PciI Enzyme

HinP1I

(G/CGC)

Ligated To

AccI (GT/CGAC), AclI, ClaI, BstBI, TaqI

AciI, BsaHI (GR/CGCC), NarI

BsaHI (GR/CGTC)

HpaII

Recleaved By

--

HhaI

HgaI

AciI

--

AciI

BanI, BsaHI, HaeII, HhaI, KasI, NarI, NlaIV

HpaII/MspI

(C/CGG)

AccI (GT/CGAC), AclI, ClaI, BstBI, TaqI

AciI, BsaHI (GR/CGCC), HinP1I, NarI

KasI

(G/GCGCC)

MfeI

(C/AATTG)

MluI

(A/CGCGT)

MseI

(T/TAA)

BanI (G/GCGCC)

ApoI (R/ATTTY), EcoRI, Tsp509I Tsp509I

AscI, BssHII BstUI, HhaI

AseI

BfaI, Csp6I, NdeI

MseI

--

--

HhaI

BanI, BsaHI, HaeII, HhaI, NarI, NlaIV

BsaHI, HgaI

AciI

FatI, NlaIII

NarI

(GG/CGCC)

AccI (GT/CGAC), AclI, ClaI, BstBI, TaqαI

AciI, HinP1I

BsaHI (GR/CGCC)

BsaHI (GR/CGTC)

HpaII

NcoI

(C/CATGG)

NdeI

(CA/TATG)

NgoMIV

(G/CCGGC)

BspHI, FatI, PciI

AseI, BfaI, Csp6I, MseI --

AgeI, BsaWI, BsrFI (R/CCGGY), SgrAI

AvaI (C/CCGGG), XmaI

BsaWI, BspEI

BsrFI (R/CCGGC), SgrAI

BsrFI, HpaII

HpaII, NciI, ScrFI

HpaII

BsrFI, Cac8I, HpaII, NaeI

BfaI NheI AvrII, SpeI, StyI (C/CTAGG), XbaI Enzyme

(G/CTAGC)

NlaIII

(CATG/)

NotI

(GC/GGCCGC)

Ligated To Recleaved By

SphI, NspI NlaIII

PspOMI

EagI

EaeI (Y/GGCCR)

AciI, EaeI, Fnu4HI, HaeIII

AciI, BsiEI, EaeI, EagI, Fnu4HI, HaeIII

AciI, BsiEI, EaeI, Fnu4HI, HaeIII

-- NsiI

(ATGCA/T)

NspI

(RCATG/Y)

PacI

(TTAAT/TAA)

PciI

(A/CATGT)

PpuMIBsiHKAI (GTGCA/C), Bsp1286I (GTGCA/C), PstI, SbfI

NlaIII, SphI NlaIII

BsiEI (CGAT/CG), PvuI MseI

BspHI, FatI, NcoI FatI, NlaIII

AvaII, RsrII

AvaII, RsrII

AvaII, RsrII

EaeI (Y/GGCCR), EagI

NotI

AvaII, Sau96I

AvaII, BsmFI, NlaIV, Sau96I

AvaII, NlaIV, Sau96I

HaeIII, Sau96I

AciI, Fnu4HI, HaeIII, Sau96I

XhoI, TliI

TaqαI

BsgI

--

PstI

DpnI, PvuI

MseI

BsiEI, DpnII, PvuI

(RG/GWCCY)

(GG/GTCCY)

(GG/GACCY)

PspOMI

(G/GGCCC)

PspXI

(VC/TCGAGB)

XhoI, TliI

SalI

PstI

(CTGCA/G)

BsiHKAI, Bsp1286I (GTGCA/C)

NsiI

SbfI

PvuI

(CGAT/CG)

AsiSI

PacI

BsiEI (CGAT/CG) Enzyme

RsrII

(CG/GWCCG)

Ligated To

AvaII, PpuMI (RG/GACCY)

PpuMI (RG/GACCY)

PpuMI (RG/GTCCY)

Recleaved By

AvaII, Sau96I

AvaII, NlaIV, Sau96I

AvaII, BsmFI, NlaIV, Sau96I

AluI, BanII, BsiHKAI, Bsp1286I, SacI SacI

(GAGCT/C)

SacII

(CCGC/GG)

SalI

(G/TCGAC)

SbfI

(CCTGCA/GG)

BanII (GAGCT/C), BsiHKAI, Bsp1286I (GAGCT/C)

BsiEI (CGGC/CG) AciI

PspXI, XhoI TaqαI

BsiHKAI, Bsp1286I (GTGCA/C)

NsiI

PstI

BsgI

--

PstI

BsgI SfcI

(C/TGCAG)

SgrAI

(CR/CCGGYG)

SpeI

(A/CTAGT)

SphI

(GCATG/C)

StyIApaLI

See BsrFI

AvrII, NheI, StyI (C/CTAGG), XbaI BfaI

NlaIII, NspI NlaIII

AvrII

NheI, SpeI, XbaI

BspHI

NcoI

AvrII, BfaI, BsaJI, StyI

BfaI

NlaIII

BsaJI, NcoI, NlaIII, StyI

TaqαI

--

Tsp509I

(C/CTAGG)

(C/CATGG)

TaqαI

(T/CGA)

AccI (GT/CGAC), ClaI, BstBI

AciI, AclI, BsaHI (GR/CGYC), HinP1I,HpaII, NarI

Tsp509I

(/AATT)

XbaI

(T/CTAGA)

ApoI (R/AATTY), EcoRI, MfeI

AvrII, NheI, SpeI, StyI (C/CTAGG) BfaI Enzyme

XhoI/TliI

(C/TCGAG)

Ligated To

PspXI

SalI

Recleaved By

XhoI, TliI

TaqαI

HpaII, NciI, ScrFI

AvaI, BsaJI, HpaII, NciI, ScrFI, SmaI, XmaI

XmaI

(C/CCGGG)

AgeI, BsaWI, BspEI, BsrFI, NgoMIV, SgrAI

AvaI (C/CCGGG)