2024年4月2日发(作者：)
目 录
中文摘要
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英文摘要
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英文缩写
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研究论文 丁苯酞对急性缺血性脑卒中认知功能及脑灌注的影响
前言
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材料与方法
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结果
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讨论
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结论
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参考文献
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综述 卒中后认知障碍的研究进展
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致谢
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个人简历
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丁苯酞对急性缺血性脑卒中认知功能及脑灌注的影响
摘 要
目的：通过建立动物缺血性脑卒中模型，从行为学、形态学及分子生
物学等多个层面，观察不同剂量丁苯酞对缺血性脑卒中大鼠认知功能及脑
灌注的影响，为临床丁苯酞治疗缺血性脑卒中提供理论依据。
方法：清洁SD大鼠25只均为雌性，体重约为250~280 g。所有大鼠
相同条件下饲养。动物由河北医科大学动物研究中心提供。将25只大鼠
从1号随机编到25号，通过随机数字表抽25个数，按从大到小顺序排列，
均建立缺血性脑卒中模型，随机分为5只为一组，共四组，分别为丁苯酞
低剂量组（A组）、丁苯酞中剂量组（B组）、丁苯酞高剂量组（C组）、
未使用丁苯酞治疗组（D组）。另5只为对照组即假手术组。行为学观察：
滚轴实验、改良大鼠神经功能缺损评分（modified Neurological Severity
Score，mNSS）；缺血灶体积的统计、脑血流量监测；分子生物学观察：
Real Time PCR检测及免疫荧光法测定Caspase-3，测定3-NT、eNOS及脑
组织NO水平。
结果：
1. 滚轴实验结果显示：在术前五组大鼠从开始至跌落下转轮的时间差
异无统计学意义。对照组即假手术组术后3天、术后7天、术后14天落
下转轮的时间与术前差异无统计学意义，其它四组手术后术后3天、术后
7天、术后14天落下转轮的时间与术前相比均下降。D组未使用丁苯酞
治疗组，术后3天、术后7天、术后14天三个时间段差异无统计学意义，
且均低于三种剂量丁苯酞组。三种剂量丁苯酞组经丁苯酞治疗后，落下转
轮的时间均有提高，即三种剂量的丁苯酞均明显促进神经功能恢复。然而，
A、B
、C三组同一时间上三组比较：术后3天，三组间差异无统计学意
义，术后7天，B组落下转轮的时间明显高于其他两组，而A、C两组间
差异无统计学意义。术后14天，B组落下转轮的时间明显高于其他两组，
而A、C两组间差异无统计学意义。同一组别不同时间比较：三组均为术
后7天、14天明显高于术后三天，A、C两组术后7天、14天落下转轮
的时间差异无统计学意义，B组术后14天落下转轮的时间明显高于术后
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