2024年4月25日发(作者：国产吸尘器十大排名)

MagMAX

™

DNA Multi-Sample Ultra 2.0 Kit

High throughput isolation of DNA from blood cards

Catalog Number A36570

Pub. No. MAN0017456 Rev. B.0

WARNING!

Read the Safety Data Sheets (SDSs) and

follow the handling instructions. Wear appropriate

protective eyewear, clothing, and gloves. Safety Data Sheets

(SDSs) are available from /support.

Product description

The Applied Biosystems

™™

Kit is developed for scalable, rapid

MagMAX DNA Multi-Sample Ultra 2.0

DNA from a variety of sample matrices. DNA

purification of high-quality

kit can be used in a broad range of molecular biology downstream

purified with this

applications, such as sequencing, genotyping, and qPCR. This

protocol guides through automated isolation of DNA from

mammalian whole blood

use with Whatman

™

Cards, using the KingFisher

903 Cards and Whatman

spotted onto blood cards, optimized for

™

™

Flex and the KingFisher

FTA

™

Classic

™

Duo Prime.

Contents and storage

Reagents provided in the kit are sufficient for 100 reactions.

Table 1 MagMAX

™

DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570)

ComponentQuantityStorage

Enhancer Solution4.5 mL

Proteinase K4.5 mL

Binding Solution45 mL

DNA Binding Beads4.5 mL

15–30°C

Wash I Solution110 mL

Elution Solution12 mL

For 1000 reaction volume use Cat. No. A36578 (Proteinase K),

A36579 (DNA Binding Beads), A36580 (Wash I Solution), A36581

(Lysis/binding Solution), A36582 (Elution Solution), and A36583

(Enhancer Solution).

For Research Use Only. Not for use in diagnostic procedures.

Required materials not supplied

Unless otherwise indicated, all materials are available through

. MLS: Fisher Scientific () or

other major laboratory supplier.

ItemSource

Magnetic particle processor (one of the following):

KingFisher

™

Particle Processor

Duo Prime Magnetic

5400110

KingFisher

™

Processor with 96 Deep-Well Head

Flex Magnetic Particle

5400630

Equipment

One of the following hole punchers:

Whatman

™

Uni-Core Punch09-924-589

Miltex

™

Biopsy PunchMLS

Incubator with metal racksMLS

Compact Digital Microplate Shaker88880023

Adjustable micropipettorsMLS

Multi-channel micropipettorsMLS

Consumables

Deep-well plates:

KingFisher

™

Deepwell 96 Plate95040450

96-well standard plates (for use with KingFisher

™

comb placement and eluate storage):

Flex only; tip

KingFisher

™

96 KF microplate97002540

Tip comb, compatible with the magnetic particle processor used:

12-tip comb, for Microtiter 96 Deepwell

plate

97003500

KingFisher

™

magnets, KingFisher

96 tip comb for DW

™

Flex protocol only

97002534

Item

Materials

MicroAmp

™

Clear Adhesive Film

One of the following blood cards:

Whatman

™

903 Protein Saver Card

Whatman

™

903 Neonatal Screening

Cards

Whatman

™

FTA Classic Cards

Source

Guidelines for Proteinase K digestion

•Do not pre-mix the Enhancer Solution and Proteinase K.

•Do not change the order of pipetting.

4306311

05-715-121

09-800-961

Guidelines for DNA Binding Bead Mix

•Vortex the DNA Binding Beads thoroughly, combine them

with the Binding Solution in a nuclease-free tube, then invert

the tube until homogeneous. This mixture can be stored for

up to 1 day before aliquoting into the plates.

•Ensure that the beads stay fully mixed within the solution

during pipetting.

•Avoid creating bubbles during mixing and aliquoting.

09-923-341 or

09-923-340

Reagents

Ethanol, 96–100% (molecular biology

grade)

Nuclease-free Water

Phosphate Buffered Saline (PBS, pH 7.4)

MLS

AM9932

10010023

Sample collection and storage

1.

Collect blood samples onto Whatman

903 Cards using a

method from the following table.

Note: A different collection volume might be needed for

other types of blood cards.

Option

Finger stick

Description

Collect samples directly on the blood cards.

™

General guidelines

•Perform all steps at room temperature (20–25°C) unless

otherwise noted.

•Precipitates and high viscosity can occur if Enhancer Solution

and Binding Solution are stored when room temperature is

too cold. If this occurs, warm them at 37°C and gently mix to

dissolve precipitates and reduce viscosity. Avoid creating

bubbles.

•Yellowing of the Binding and Wash I Solution is normal and

will not affect buffer performance

•Cover the plate during the incubation and shaking steps to

prevent spill-over and cross-contamination. The same

™

MicroAmp Clear Adhesive Film can be used throughout the

procedure, unless it becomes contaminated.

•If using a plate shaker other than the recommended shaker,

verify that:

plate fits securely on the plate shaker.

recommended speeds are compatible with the plate

shaker (Ideal shaker speeds allow for thorough mixing

without splashing).

•Per-plate volumes for reagent mixes are sufficient for one

plate plus overage. To calculate volumes for other sample

numbers, refer to the per-well volume and add 10% overage.

•(Optional) To prevent evaporation and contamination, cover

the prepared processing plates with paraffin film or

™

MicroAmp Clear Adhesive Film until they are loaded into

the instrument.

VenipunctureCollect samples in EDTA or sodium citrate

anticoagulant tubes, then transfer to blood

cards.

IMPORTANT!

Heparin is not recommended as an anti-

coagulant as it can cause inhibition of PCR reactions.

2.

Dry the cards at room temperature for at least 3 hours, or

according to the manufacturers' instructions.

Lay cards on a flat surface and allow to completely air dry.

3.

Process samples shortly after they are completely dry or,

store the cards at room temperature with a desiccant packet

or in a humidity-controlled, cool, and dry environment.

Before first use of the kit

Prepare Wash II Solution: Make 80% ethanol from 100% absolute

ethanol and Nuclease-Free Water.

Before each use of the kit

Vortex DNA Binding Beads to fully resuspend the beads before

each use.

Prepare samples and digest with Proteinase K

1

Prepare samples and

digest with Proteinase K

Preheat an incubator to 65°C.

a.

Cut out the blood cards using a Whatman

Uni-Core punch or a Miltex biopsy punch.

b.

Prepare 2-, 3-, or 4-mm sized discs, using 1 or 2 discs per reaction, then transfer them to the

appropriate wells of the Proteinase K 96-well DW Plate using forceps.

™™

2

MagMAX

™

DNA Multi-Sample Ultra 2.0 Kit User Guide (blood cards)