2024年3月23日发(作者：努比亚z11拆机视频)

Alexa Fluor 488标记山羊抗兔IgG (H+L)

产品编号

A0423

产品名称

Alexa Fluor 488标记山羊抗兔IgG (H+L)

包装

100μl

产品简介：

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本Alexa Fluor 488标记山羊抗兔IgG (H+L) (Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H+L))为进口分装，用于免疫荧光

染色。

Alexa Fluor 488是一种常用的非常明亮的绿色荧光探针。它比绝大部分常用的绿色荧光探针更加明亮，更加不容易淬灭，

而且背景更低。Alexa Fluor 488的荧光光谱与FITC和Cy2比较接近。Alexa Fluor 488的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)

Alexa Fluor 488 495 519

本抗体为用纯化的兔IgG免疫山羊，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人IgG、小鼠IgG和大鼠IgG吸附

纯化的优质二抗。对人IgG、小鼠IgG和大鼠IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染

色实验。

本Alexa Fluor 488标记山羊抗兔IgG (H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原

和抗体的具体情况适当调节荧光标记二抗的稀释比例，推荐的调节范围为1:200-1000。

本抗体如果用于常规的免疫染色，以每次检测需1毫升1:500稀释的荧光标记二抗计，至少可以检测50次。如果适当重复使

用已经使用过的荧光标记二抗，至少可以多检测150-250次。

产品编号

A0423

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产品名称

Alexa Fluor 488标记山羊抗兔IgG (H+L)

说明书

包装

100μl

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包装清单：

1份

保存条件：

-20℃避光保存，一年有效。

注意事项：

 为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：

1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。

2. 如果希望重复使用稀释的荧光标记二抗，稀释的荧光标记二抗4℃保存。

使用本产品的文献：

1. Lu X, Zhang N, Meng B, Dong S, Hu Y.

Involvement of GPR12 in the regulation of cell proliferation and survival.

Mol Cell Biochem. 2012 Jul;366(1-2):101-10.

2. Lu X, Zhang N, Dong S, Hu Y.

Involvement of GPR12 in he induction of neurite outgrowth in PC12 cells.

Brain Res Bull. 2012 Jan 4;87(1):30-6.

3. Su J, Wang Y, Li R, Peng H, Hua S, Li Q, Quan F, Guo Z, Zhang Y.

Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo development of SCNTembryos in cattle.

PLoS One. 2012;7(4):e36181. doi: 10.1371/.0036181. Epub 2012 Apr 27.

4. Qiu M, Quan F, Han C, Wu B, Liu J, Yang Z, Su F, Zhang Y.

Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal cells and theca cells derivedfrom the goat ovary.

J Steroid Biochem Mol Biol. 2013 Jun 28. pii: S0960-0760(13)00120-9. doi: 10.1016/.2013.06.005.

5. Yang Z, Liu J, Liu H, Qiu M, Liu Q, Zheng L, Pang M, Quan F, Zhang Y.

Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat Skin Fibroblasts.

Cell Reprogram. 2013 Jun;15(3):195-205. doi: 10.1089/cell.2012.0080. Epub 2013 May 13.

6. Liu J, Yang Z, Qiu M, Luo Y, Pang M, Wu Y, Zhang Y.

Developmental potential of cloned goat embryos from an SSEA3(+) subpopulation of skin fibroblasts.

Cell Reprogram. 2013 Apr;15(2):159-65. doi: 10.1089/cell.2012.0073. Epub 2013 Feb 26.

7. Deng Z, Yan F, Jin Q, Li F, Wu J, Liu X, Zheng H.

Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome-microbubble complexes assisted by ultrasound.

J Control Release. 2014 Jan 28;174:109-16. doi: 10.1016/l.2013.11.018. Epub 2013 Nov 25.

8. Miao SH, Sun HB, Ye Y, Yang JJ, Shi YW, Lu M, Hu G, Zhou JW.

Astrocytic JWA Expression is Essential to Dopaminergic Neuron Survival in the Pathogenesis of Parkinson's Disease.

CNS Neurosci Ther. 2014 Aug;20(8):754-62. doi: 10.1111/cns.12249. Epub 2014 Mar 17.

9. Su J, Hu G, Wang Y, Liang D, Gao M, Sun H, Zhang Y.

Recombinant human growth differentiation factor-9 improves oocyte reprogramming competence and subsequent development of bovine cloned embryos.

Cell Reprogram. 2014 Aug;16(4):281-9. doi: 10.1089/cell.2014.0001. Epub 2014 May 19.

ZW, Pan DD, Wu Z, Sun YY, Guo YX, Zeng XQ.

Antialcoholic liver activity of whey fermented by Lactobacillus casei isolated from koumiss.

J Dairy Sci. 2014 Jul;97(7):4062-71. doi: 10.3168/jds.2014-7954. Epub 2014 Apr 24.

Y, Sun C, Feng L, Li P, Xiao L, Ren Y, Wang D, Li C, Chen L.

Regulation of SIV antigen-specific CD4+ T cellular immunity via autophagosome-mediated MHC II molecule-targeting antigen presentation in mice.

PLoS One. 2014 Mar 26;9(3):e93143. doi: 10.1371/.0093143. eCollection 2014.

H, Xiao Y, Wang X, Riaz H, Li W, Fu S, Xin Y, Shi L, Ma F, Li X, Yang L.

Effects of histone deacetylase inhibitors on the early development of bovine androgenetic embryos.

Cell Reprogram. 2014 Feb;16(1):54-64. doi: 10.1089/cell.2013.0027. Epub 2014 Jan 4.

W, Gu Y, Li Z, Cai H, Peng Q, Tu M, Kondo Y, Shinjo K, Zhu Y, Zhang J, Sekido Y, Han B, Qian Z, Miao Y.

miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in pancreatic ductal adenocarcinoma.

Oncogene. 2014 Apr 28;0. doi: 10.1038/onc.2014.101.

J, Sun Y, Zhang X, Zhang F, Zhang S, Yu S, Qiu X, Tan L, Song C, Gao S, Wu Y, Ding C.

Toll-like receptor 3 inhibits Newcastle disease virus replication through activation of pro-inflammatory cytokines and the type-1 interferon pathway.

Arch Virol. 2014 Jun 17.

J, Wang Y, Zhang L, Wang B, Liu J, Luo Y, Guo Z, Quan F, Zhang Y.

Oocyte-secreted factors in oocyte maturation media enhance subsequent development of bovine cloned embryos.

Mol Reprod Dev. 2014 Apr;81(4):341-9. doi: 10.1002/mrd.22302. Epub 2014 Feb 18.

XY, Yang LQ, Jiang Y, Yang WW, Fu J, Li SL.

Reactive oxygen species and autophagy associated apoptosis and limitation of clonogenic survival induced by zoledronic acid in salivary adenoid cystic

carcinoma cell line SACC-83.

PLoS One. 2014 Jun 25;9(6):e101207. doi: 10.1371/.0101207. eCollection 2014.

J, Wang Y, Li W, Gao M, Ma Y, Hua S, Quan F, Zhang Y.

Effects of 3-hydroxyflavone on the cellular and molecular characteristics of bovine embryos produced by somatic-cell nuclear transfer.

Mol Reprod Dev. 2014 Mar;81(3):257-69. doi: 10.1002/mrd.22293. Epub 2014 Jan 10.