2024年3月10日发(作者：)

Advances in Clinical Medicine 临床医学进展, 2019, 9(6), 807-814

Published Online June 2019 in Hans. /journal/acm

/10.12677/acm.2019.96124

Experimental Study on the Activity of

Acetylcholinesterase (chAT) and

Acetylcholinesterase (a-che) in

the Brain of Rats with Alzheimer’s Disease

Qijun Long

1

, Yuying Deng

1

, Chuanling Tan

1

, Dan Zou

1

, Shumei Xu

1

, Haichao Tian

1

,

Shuqiu Zhang

2*

, Guoquan Zhou

3

1

2

Heavy Metal and Arsenic Toxicology Research Laboratory, Youjiang University of Nationalities, Baise Guangxi

Guangxi Baise High-Tech Zone Science and Technology Enterprise Incubation Base R & D Center, Baise Guangxi

3

Department of Environmental Toxicology, Michigan State University, East Lansing, Michigan, USA

ththth

Received: Jun. 11

, 2019; accepted: Jun. 20, 2019; published: Jun. 27, 2019

Abstract

Objective: To explore the effect of Chinese herbal medicine and compound preparation on mice of

aluminum-induced Alzheimer's disease (AD). Methods: AD mice model was established first. 50

mice were divided into four groups: control group, model group, treatment group 1 and treatment

group 2. Except the control group mice, the model group mice were injected intraperitoneally with

mixed liquid of D-galactose + AlCl

3

for 60 days (the mice were injected intraperitoneally with Al

3+

concentration of 2 mg/ml aluminum trichloride diluted liquid at a dose of 5 mg/kg body weight

for 60 days). Treatment group 1 and treatment group 2 were poisoned for 2 months with alumi-

num first, then the two treatment groups were poured with different dosages of Chinese herbal

compound preparations respectively; the same volume of distilled water was given to mice of the

control group and model group till to the end of the experiment. Hemoglobin and Morris water

maze test were tested before and after experiment. At the end of experiment, the blood was ob-

tained, the serum was isolated, and serum biochemical indexes were measured. After mice sacri-

ficed, their brains were taken and weighed, then the brains were made into homogenate, and cen-

trifuged to get the supernatant, in which acetylcholinesterase (AchE), acetylcholine transferase



) cleaning rate, and glutathione were determined. And

(chAT), superoxide anion free radical (

O2

another part of the brain was used for pathological examination after formaldehyde treatment.

Results: In all experimental groups, the indices showed respectively that vitality of mice’s brain

(AchE) was 5.77 ± 1.52, 6.02 ± 0.79, 7.30 ± 0.59, 5.27 ± 1.09 (U/), P < 0.05, P < 0.01; Vigor

Dynamic of (ChAT) was 29.25 ± 13.42, 7.05 ± 5.07, 52.95 ± 25.79, 53.95 ± 12.82 (U/g) (tissue wet

weight), P < 0.05, P < 0.01; and Vigor Dynamic of serum (AchE): 51.79 ± 2.12, 44.71 ± 2.21, 55.41 ±

2.10, 41.30 ± 3.36 (U/ml), P < 0.05, P < 0.01; obviously, in all these indexes, the model group was

apparently lower. Al

3+

in mice’s brain content turned out 135.00 ± 8.37, 149.40 ± 0.89, 147.43 ±



) cleaning rate was 27.65 ± 4.81,

4.83, 118.75 ± 6.41 (ng/ml); superoxide anion free radical (

O2

14.71 ± 3.60, 22.65 ± 8.67, 21.57 ± 6.14 (%). Before, during, after the contamination, it showed

*

通讯作者。

文章引用: 龙奇军, 邓钰莹, 谭川铃, 邹丹, 许淑妹, 田海潮, 张树球, 周国荃. 阿尔茨海默病模型小鼠脑乙酰胆碱转

移酶(chAT)和乙酰胆碱酯酶(A-chE)活力的实验研究[J]. 临床医学进展, 2019, 9(6): 807-814.

DOI: 10.12677/acm.2019.96124

龙奇军 等

that Hb in the control group had no obvious change, in model group was low, and in the treatment

group was lower first then increased. Conclusion: Aluminum can induce the brain neuron cell suf-

fering from atrophy, degeneration and necrosis, which will reduce the AchE activity, chAT activi-

ties, and the anti-oxidative ability. Chinese medicine compound preparations have obvious cura-

tive effect on AD through excreting aluminum and improving anti-oxidative ability.

Keywords

Aluminum, Alzheimer’s Disease, Ache, Antioxidant Ability

阿尔茨海默病模型小鼠脑乙酰胆碱转移酶(chAT)

和乙酰胆碱酯酶(A-chE)活力的实验研究

龙奇军

1

，邓钰莹

1

，谭川铃

1

，邹 丹

1

，许淑妹

1

，田海潮

1

，张树球

2*

，周国荃

3

1

2

右江民族医学院重金属与氟砷毒物研究实验室，广西 百色

广西百色高新区科技企业孵化基地研发中心，广西 百色

3

美国密西根州立大学环境科学毒理学教研室，兰辛，密西根州，美国

收稿日期：2019年6月11日；录用日期：2019年6月20日；发布日期：2019年6月27日

摘 要

目的：探讨中药茯苓及复方制剂对铝致老年痴呆症(AD)小鼠治疗效果的观察及对乙酰胆碱转移酶(chAT)，

乙酰胆碱酯酶(AchE)的影响。方法：建立铝致AD小鼠动物模型，将50只小鼠分为正常组、模型组、治

疗1组和治疗2组；除正常组外，造模组用D-半乳糖 + AlCl

3

混合水溶液腹腔注射(Al

3+

+ 浓度2 mg/ml的

三氯化铝水溶液按5 mg/kg体重剂量给小鼠稀释后腹腔注射60天)。治疗1、2组在铝染毒2个月后同时给

予中药复方制剂不同剂量灌胃，模型组和正常组用等量体积蒸馏水灌胃，直至实验结束。在实验前、后

分别进行水迷宫游水试验，并测定血红蛋白；实验结束后取血，分离血清，测定血清生化指标；处死小

鼠取脑，称脑重量，制成脑匀浆，离心取上清，分别测定脑中乙酰胆碱酯酶(AchE)、乙酰胆碱转移酶(chAT)、



)清除率、

超氧阴离子自由基(

O2

谷胱甘肽等含量；另部分脑用甲醛处理后作病理检查。结果：大脑(AchE)

活力，正常组、模型组、治1组、治2组依次为，5.77 ± 1.52、6.02 ± 0.79、7.30 ± 0.59、5.27 ± 1.09

▲▲▲

(U/ )

，与治疗1组比较，P < 0.05，P < 0.01，差异有统计学意义；治疗1组明显升高；脑乙

★▲▲★★▲

酰胆碱转移酶(chAT)活力依次为，29.25 ± 13.42、7.05 ± 5.07、52.95 ± 25.79、53.95 ± 12.82

★★▲★★★

(U/g组织湿重)，与正常组比较，P < 0.05，与模型组比较，P < 0.05，P < 0.01，差异有统计学

▲▲★★

意义；模型组明显降低；血清乙酰胆碱酯酶(AchE)活力依次为51.79 ± 2.12、44.71 ± 2.21、55.41 ±

★▲▲★★▲▽▽★★★

2.10

、41.30 ± 3.36

(U/ml)，与正常组比较，P < 0.05，P < 0.01，差异有统计学意义；与

▲▲▲▽▽

模型组比较，

P < 0.05，P < 0.01，差异有统计学意义；与治疗1组比较，P < 0.01，差异有统计学

意义；模型组明显降低；脑铝Al

3+

含量依次为135.00 ± 8.37、149.40 ± 0.89、147.43 ± 4.83、118.75 ±



)清除率依次为27.65 ± 4.81、14.71 ± 3.60、22.65 ± 8.67、21.57

6.41 (ng/ml)

，超氧阴离子自由基(

O2

▲▲▲

± 6.14 (%)

，造模前、中、后，Hb依次为正常组无明显变化、模型组降低、治疗组先降低后升高。结论：

铝可导致大脑chAT下降、AchE活性升高，乙酰胆碱减少，胆碱能神经障碍，认知功能障碍，可能是老

年痴呆症发病的重要原因，同时铝又使动物抗氧化能力降低，加重疾病；本中药复方制剂通过排铝、恢

DOI: 10.12677/acm.2019.96124

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临床医学进展